Countstar Mira HT – High-Throughput Cell Analyzer
The Countstar Mira HT is a high-throughput automated cell analyzer designed for high-volume cell analysis workflows in research and regulated biomanufacturing environments. Integrating AI-learning algorithms, advanced imaging technology, and broad automation compatibility, it delivers fast, accurate, and reproducible results at scale.
Key Features
- 8.3 MP CMOS camera with 5x high NA objective providing excellent optical resolution and high fluorescence sensitivity
- Expanded field of view twice that of a standard hemocytometer, improving counting accuracy especially for diluted samples
- Patented fixed-focus technology eliminates manual focusing errors for consistent, reproducible results
- High throughput — automatically analyzes 15 to 24 samples in a single run using the proprietary 24-chamber plate, completable in under 5 minutes
- Fast image acquisition — Trypan Blue analysis completed in under 20 seconds; AO/PI or GFP assays in 60 seconds
- Automation compatible — linkable to automated liquid handling platforms and slide storage stations for fully customized automated workflows
- AI-learning algorithms capable of identifying wide range of cell sizes, irregular morphologies, and cultures with tendency to form clusters
- FDA 21 CFR Part 11 and cGMP compliant with electronic signatures, audit trail, multi-level user privileges, data encryption, and complete 3Q validation services
- 1 TB internal storage with real-time LAN-based data transfer to central servers, exportable in CSV, PDF, and image formats
- 8-inch HD touchscreen with BioApp-structured result database for intuitive data retrieval and management
Applications
- Trypan Blue viability assay — high-throughput cell concentration and viability analysis validated on CHO, 293T, and MCF-7 cells; results comparable to hemocytometer with superior reproducibility (CV < 7%, R² = 0.9955)
- AO/PI dual fluorescence viability — advanced concentration and viability measurement effective even in the presence of impurities or anucleate cells; validated on PBMC, CHO, MSC, Human T-cells, RAW264.7, and Zebrafish cells (R² = 0.9943, CV < 5%)
- GFP/RFP transfection efficiency — accurate quantification of transfection efficiency for product yield monitoring and viral vector optimization in cell and gene therapy; validated on GFP-transfected HEK293 cells with results comparable to flow cytometry (CytoFlex)
- Specialty object counting — iPSC cell cluster counting and immunomagnetic bead residue quantification using the proprietary 24-chamber plate
- Growth curve monitoring — automated generation of growth curves and diameter distribution histograms for longitudinal tracking of cell culture kinetics across multiple cultivation runs