Countstar Mira FL Plus – Automated Fluorescence Cell Analyzer

The Countstar Mira FL Plus is an advanced fluorescence cell analyzer that integrates AI deep-learning algorithms and patented fixed-focus technology to deliver accurate, reproducible, and regulation-compliant cell analysis. Designed for both routine and complex assays, it is purpose-built for research laboratories and regulated pharmaceutical and biomanufacturing environments.

Key Features

• 8.3 MP cooled CMOS color camera with high-performance optical lens providing high fluorescence sensitivity and detail-rich, high-resolution images
• Patented fixed-focus technology eliminates manual focusing errors and ensures consistent, reproducible results with CV < 5% across multiple instruments
• Expanded field of view approximately twice that of a standard hemocytometer, improving statistical accuracy and counting precision
• AI deep-learning algorithms trained across different backgrounds, focal lengths, and sample characteristics for accurate identification of cells with irregular morphology, aggregation, or uneven sizes
• Customizable BioApps — comprehensive suite of pre-installed assay templates expandable for new and challenging applications
• Multi-dimensional data analysis automatically generating growth curves, diameter distribution maps, and fluorescence distribution maps
• FDA 21 CFR Part 11 and cGMP compliant with full audit trail, password-protected user login, multi-level user privileges, electronic signatures, and complete 3Q validation support
• Flexible data backup via network printer or local server in real time, with manual or scheduled automatic backup options
• 128 GB internal storage with export options in CSV, PDF, and JPEG formats
• Compact and lightweight at 4 kg and 240 × 220 × 285 mm with a glove-sensitive 8-inch HD touchscreen

Applications

• AO/PI dual fluorescence viability — advanced cell density and viability analysis validated on PBMCs (whole blood compatible) and CHO cells; R² = 0.9993, CV < 5%
• Trypan Blue viability assay — precise cell titer and viability for CHO, MSC, and MCF-7 cells, including aggregated and irregularly shaped cells; R² = 0.9995, CV < 5%
• GFP/RFP transfection efficiency — quantification of transfection efficiency for gene therapy and viral vector workflows; validated on GFP-transfected HEK293 cells with results comparable to flow cytometry
• Complex cell morphology analysis — AI-powered identification of MSC cells with irregular morphology, RAW264.7 cells prone to aggregation, and freshly digested zebrafish embryonic cells with uneven sizes
• Cell and gene therapy process development — streamlined workflows for CAR-T, NK cell, and viral vector-based process development and optimization
• Growth curve monitoring — longitudinal tracking of cell culture kinetics with automatic curve generation for comparing multiple cultivations